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Product Info Summary
| SKU: | LS002547 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6) |
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Product info
Product Name
Ku70 (XRCC6) (NM_001469) Human Over-expression Lysate
SKU/Catalog Number
LS002547
Size
100 µg
Description
Transient overexpression lysate of X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6)
Storage & Handling
Cite This Product
Ku70 (XRCC6) (NM_001469) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS002547)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Ku70 (XRCC6) Human Over-expression Lysates NM_001469.
Western blot validation of overexpression lysate (LS002547) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with XRCC6 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for XRCC6 (Source: Uniprot.org, NCBI)
Gene Name
XRCC6
Full Name
X-ray repair cross-complementing protein 6
Weight
69.843kDa
Superfamily
ku70 family
Alternative Names
5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; D22S731; EC 3.6.4.-; EC 4.2.99.-; G22P1; G22P1Ku70; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; Ku70; ML8; ML8,70 kDa subunit; thyroid-lupus autoantigen p70; Thyroid-lupus autoantigen; TLAA; TLAAD22S671; X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6; X-ray repair cross-compleme XRCC6 CTC75, CTCBF, G22P1, KU70, ML8, TLAA X-ray repair cross complementing 6 X-ray repair cross-complementing protein 6|5-dRP lyase Ku70|5-deoxyribose-5-phosphate lyase Ku70|70 kDa subunit of Ku antigen|ATP-dependent DNA helicase 2 subunit 1|ATP-dependent DNA helicase II, 70 kDa subunit|CTC box binding factor 75 kDa subunit|DNA repair protein XRCC6|Ku autoantigen p70 subunit|Ku autoantigen, 70kDa|X-ray repair complementing defective repair in Chinese hamster cells 6|lupus Ku autoantigen protein p70|thyroid autoantigen 70kD (Ku antigen)|thyroid autoantigen 70kDa (Ku antigen)|thyroid-lupus autoantigen p70
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on XRCC6, check out the XRCC6 Infographic
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In this infographic, you will see the following information for XRCC6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Ku70 (XRCC6) (NM_001469) Human Over-expression Lysate (LS002547)
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